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  • bovine serum albumin elisa 1

BlueGene Biotech Human Oxidized lowdensity lipoprotein ELISA kit

E01O0341 Human Oxidized lowdensity lipoprotein ELISA kit

The Human Oxidized lowdensity lipoprotein ELISA kit can be used to identify samples from the human species. Oxidized lowdensity lipoprotein can also be called Ox-LDL.

Specifications of Human Oxidized lowdensity lipoprotein ELISA kit

Product Information

Cat. No.

E01O0341

Product Name

Human Oxidized lowdensity lipoprotein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OxLDL ELISA kit uses an anti-OxLDL antibody and an OxLDL-HRP conjugate in a competitive enzyme immunoassay method. OxLDL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OxLDL antibody binding site between OxLDL from samples and OxLDL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OxLDL. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve.


Quality Control on Human Oxidized lowdensity lipoprotein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OxLDL and analogues was observed.


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Summary of the Assay Procedure for Human Oxidized lowdensity lipoprotein ELISA kit

Summary of the Assay Procedure for Human Oxidized lowdensity lipoprotein ELISA kit

Citations of Human Oxidized lowdensity lipoprotein ELISA kit

E01O0341 has been referenced in the below publications:

THE VAIUATION OF CXCL16 AND ITS CLINICAL SIGNIFICANCE IN CHILDREN WITH PRIMARY NEPHROTIC SYNDROME.

The role of Caveolin-1/NF-kB/HMGBl signal pathway in OX-LDL-induced endothelial injury and the protective effect of probucol on endothelial progenitor cells in hyperlipidemia.

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