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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Cluster of Differentiation 74 ELISA kit (E01C0206)

E01C0206 Human Cluster of Differentiation 74 ELISA kit

The Human Cluster of Differentiation 74 ELISA kit can be used to identify samples from the Human species. Cluster of Differentiation 74 can also be called CD74, DHLAG, HLADG, II, Ia-GAMMA, CD74 molecule, p33, CLIP.

Products

Specifications of Human Cluster of Differentiation 74 ELISA kit

Product Information

Cat. No.

E01C0206

Product Name

Human Cluster of Differentiation 74 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CD74 ELISA kit uses an anti-CD74 antibody and an CD74-HRP conjugate in a competitive enzyme immunoassay method. CD74-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD74 antibody binding site between CD74 from samples and CD74-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CD74. Since the number of sites is limited, as more sites are occupied by CD74 from the sample, fewer sites are left to bind CD74-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD74 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Cluster of Differentiation 74 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD74 and analogues was observed.


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Summary of the Assay Procedure for Human Cluster of Differentiation 74 ELISA kit

Summary of the Assay Procedure for Human Cluster of Differentiation 74 ELISA kit

Citations of Human Cluster of Differentiation 74 ELISA kit

E01C0206 has been referenced in the below publications:

NF-KB抑制剂对宫颈癌细胞株生物学特性的影响.

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