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  • bovine serum albumin elisa 1

BlueGene Biotech Human Connective Tissue Growth Factor ELISA kit

E01C0563 Human Connective Tissue Growth Factor ELISA kit

The Human Connective Tissue Growth Factor ELISA kit can be used to identify samples from the human species. Connective Tissue Growth Factor can also be called CCN2, HCS24, IGFBP8, NOV2, connective tissue growth factor, cellular communication network factor 2, CTGF, CCN family member 2, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8.

Specifications of Human Connective Tissue Growth Factor ELISA kit

Product Information

Cat. No.

E01C0563

Product Name

Human Connective Tissue Growth Factor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CTGF ELISA kit uses an anti-CTGF antibody and an CTGF-HRP conjugate in a competitive enzyme immunoassay method. CTGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTGF antibody binding site between CTGF from samples and CTGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTGF. Since the number of sites is limited, as more sites are occupied by CTGF from the sample, fewer sites are left to bind CTGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTGF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Connective Tissue Growth Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CTGF and analogues was observed.


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Summary of the Assay Procedure for Human Connective Tissue Growth Factor ELISA kit

Summary of the Assay Procedure for Human Connective Tissue Growth Factor ELISA kit

Citations of Human Connective Tissue Growth Factor ELISA kit

E01C0563 has been referenced in the below publications:

2型糖尿病患者心肌纤维化血清标志物。

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