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BlueGene Biotech Human Fibroblast Growth Factor 21 ELISA kit (E01F0016)

E01F0016 Human Fibroblast Growth Factor 21 ELISA kit

The Human Fibroblast Growth Factor 21 ELISA kit can be used to identify samples from the human species. Fibroblast Growth Factor 21 can also be called FGF 21, FGF21, fibroblast growth factor 21, FGF-21.


Products

Specifications of Human Fibroblast Growth Factor 21 ELISA kit

Product Information

Cat. No.

E01F0016

Product Name

Human Fibroblast Growth Factor 21 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FGF 21 ELISA kit uses an anti-FGF 21 antibody and an FGF 21-HRP conjugate in a competitive enzyme immunoassay method. FGF 21-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FGF 21 antibody binding site between FGF 21 from samples and FGF 21-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FGF 21. Since the number of sites is limited, as more sites are occupied by FGF 21 from the sample, fewer sites are left to bind FGF 21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF 21 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Fibroblast Growth Factor 21 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FGF 21 and analogues was observed.


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Summary of the Assay Procedure for Human Fibroblast Growth Factor 21 ELISA kit

Summary of the Assay Procedure for Human Fibroblast Growth Factor 21 ELISA kit

Citations of Human Fibroblast Growth Factor 21 ELISA kit

E01F0016 has been referenced in the below publications:

Changes and its influence factor of serum fibroblast growth factor 21 levels in patients with thyroid dysfunction.

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