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BlueGene Biotech Human Heat Shock Protein 27 Antibody ELISA kit

E01H0290 Human Heat Shock Protein 27 Antibody ELISA kit

The Human Heat Shock Protein 27 Antibody ELISA kit can be used to identify samples from the human species. Heat Shock Protein 27 Antibody can also be called HSPB1-ab, CMT2F-ab, HEL-S-102-ab, HMN2B--ab, HS.76067-ab, HSP27-ab, HSP28-ab, Hsp25-ab, SRP27-ab, heat shock protein family B (small) member 1 antibody.

Specifications of Human Heat Shock Protein 27 Antibody ELISA kit

Product Information

Cat. No.

E01H0290

Product Name

Human Heat Shock Protein 27 Antibody ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

0.5-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HSP27-Ab ELISA kit uses an anti-HSP27-Ab antibody and an HSP27-Ab-HRP conjugate in a competitive enzyme immunoassay method. HSP27-Ab-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HSP27-Ab antibody binding site between HSP27-Ab from samples and HSP27-Ab-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HSP27-Ab. Since the number of sites is limited, as more sites are occupied by HSP27-Ab from the sample, fewer sites are left to bind HSP27-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP27-Ab concentration in each sample is interpolated from this standard curve.


Quality Control on Human Heat Shock Protein 27 Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HSP27-Ab and analogues was observed.


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Summary of the Assay Procedure for Human Heat Shock Protein 27 Antibody ELISA kit

Summary of the Assay Procedure for Human Heat Shock Protein 27 Antibody ELISA kit

Citations of Human Heat Shock Protein 27 Antibody ELISA kit

E01H0290 has been referenced in the below publications:

To explore the relationship of the level of serum anti-HSP27 and the presence and severity of coronary artery disease.

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