E01I0396 Human Inducible nitric oxide synthase ELISA kit
The Human Inducible nitric oxide synthase ELISA kit can be used to identify samples from the human species. Inducible nitric oxide synthase can also be called I NOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, Inosl, MAC NOS, Macrophage NOS.
Product Information | |
Cat. No. | E01I0396 |
Product Name | Human Inducible nitric oxide synthase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
iNOS ELISA kit uses an anti-iNOS antibody and an iNOS-HRP conjugate in a competitive enzyme immunoassay method. iNOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-iNOS antibody binding site between iNOS from samples and iNOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of iNOS. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between iNOS and analogues was observed. | |
E01I0396 has been referenced in the below publications：
Mechanism of Rosamultin on inhabiting Ca2+ and apoptosis of EA.hy926 endothefial cells induced by hypoxia.
The role of combined detection of iNOS mRNA-iNOS and NO in the treatment and the prognosis decision of AR.
Macrophages of the Related Factor in the Clinical Value of Tuberculosis.
Sildenafil Protects against Myocardial Ischemia-Reperfusion Injury Following Cardiac Arrest in a Porcine Model: Possible Role of the Renin-Angiotensin System.
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