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BlueGene Biotech Human Inducible nitric oxide synthase ELISA kit (E01I0396)

E01I0396 Human Inducible nitric oxide synthase ELISA kit

The Human Inducible nitric oxide synthase ELISA kit can be used to identify samples from the human species. Inducible nitric oxide synthase can also be called I NOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS, Inosl, MAC NOS, Macrophage NOS.

Products

Specifications of Human Inducible nitric oxide synthase ELISA kit

Product Information

Cat. No.

E01I0396

Product Name

Human Inducible nitric oxide synthase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

iNOS ELISA kit uses an anti-iNOS antibody and an iNOS-HRP conjugate in a competitive enzyme immunoassay method. iNOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-iNOS antibody binding site between iNOS from samples and iNOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of iNOS. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.


Quality Control on Human Inducible nitric oxide synthase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between iNOS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Inducible nitric oxide synthase ELISA kit

Summary of the Assay Procedure for Human Inducible nitric oxide synthase ELISA kit

Citations of Human Inducible nitric oxide synthase ELISA kit

E01I0396 has been referenced in the below publications:

Mechanism of Rosamultin on inhabiting Ca2+ and apoptosis of EA.hy926 endothefial cells induced by hypoxia.

The role of combined detection of iNOS mRNA-iNOS and NO in the treatment and the prognosis decision of AR.

Macrophages of the Related Factor in the Clinical Value of Tuberculosis.

Sildenafil Protects against Myocardial Ischemia-Reperfusion Injury Following Cardiac Arrest in a Porcine Model: Possible Role of the Renin-Angiotensin System.

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