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BlueGene Biotech Human Interleukin 17A ELISA kit

E01I0362 Human Interleukin 17A ELISA kit

The Human Interleukin 17A ELISA kit can be used to identify samples from the human species. Interleukin 17A can also be called IL 17A, IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A, ILA17, Interleukin 17A.

Specifications of Human Interleukin 17A ELISA kit

Product Information

Cat. No.

E01I0362

Product Name

Human Interleukin 17A ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL17A ELISA kit uses an anti-IL17A antibody and a IL17A-HRP conjugate in a competitive enzyme immunoassay method. IL17A-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL17A antibody binding site between IL17A from samples and IL17A-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL17A. Since the number of sites is limited, as more sites are occupied by IL17A from the sample, fewer sites are left to bind IL17A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL17A concentration in each sample is interpolated from this standard curve.


Quality Control on Human Interleukin 17A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL17A and analogues was observed.


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Summary of the Assay Procedure for Human Interleukin 17A ELISA kit

Summary of the Assay Procedure for Human Interleukin 17A ELISA kit

Citations of Human Interleukin 17A ELISA kit

E01I0362 has been referenced in the below publications:

Detection of Th17/Treg Cell-associated Cytokines in Peripheral Blood of Patients with Vitiligo and its Clinical Sig-nificance.

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