E01A0653 Human 12 Hydroxyeicosatetraenoic acid ELISA kit
The Human 12 Hydroxyeicosatetraenoic acid ELISA kit can be used to identify samples from the human species. 12 Hydroxyeicosatetraenoic acid can also be called 12 HETE, 12-HETE, 12(S)-HETE.
Specifications of Human 12 Hydroxyeicosatetraenoic acid ELISA kit
Product Information | |
Cat. No. | E01A0653 |
Product Name | Human 12 Hydroxyeicosatetraenoic acid ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
12 HETE ELISA kit uses an anti-12 HETE antibody and an 12 HETE-HRP conjugate in a competitive enzyme immunoassay method. 12 HETE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-12 HETE antibody binding site between 12 HETE from samples and 12 HETE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 12 HETE. Since the number of sites is limited, as more sites are occupied by 12 HETE from the sample, fewer sites are left to bind 12 HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 12 HETE concentration in each sample is interpolated from this standard curve. |
Quality Control on Human 12 Hydroxyeicosatetraenoic acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 12 HETE and analogues was observed. | |
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Summary of the Assay Procedure for Human 12 Hydroxyeicosatetraenoic acid ELISA kit
Citations of Human 12 Hydroxyeicosatetraenoic acid ELISA kit
E01A0653 has been referenced in the below publications：
Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.
