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BlueGene Biotech Human 12 Hydroxyeicosatetraenoic acid ELISA kit (E01A0653)

E01A0653 Human 12 Hydroxyeicosatetraenoic acid ELISA kit

The Human 12 Hydroxyeicosatetraenoic acid ELISA kit can be used to identify samples from the human species. 12 Hydroxyeicosatetraenoic acid can also be called 12 HETE, 12-HETE, 12(S)-HETE.

Products

Specifications of Human 12 Hydroxyeicosatetraenoic acid ELISA kit

Product Information

Cat. No.

E01A0653

Product Name

Human 12 Hydroxyeicosatetraenoic acid ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

12 HETE ELISA kit uses an anti-12 HETE antibody and an 12 HETE-HRP conjugate in a competitive enzyme immunoassay method. 12 HETE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-12 HETE antibody binding site between 12 HETE from samples and 12 HETE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 12 HETE. Since the number of sites is limited, as more sites are occupied by 12 HETE from the sample, fewer sites are left to bind 12 HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 12 HETE concentration in each sample is interpolated from this standard curve.


Quality Control on Human 12 Hydroxyeicosatetraenoic acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 12 HETE and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human 12 Hydroxyeicosatetraenoic acid ELISA kit

Summary of the Assay Procedure for Human 12 Hydroxyeicosatetraenoic acid ELISA kit

Citations of Human 12 Hydroxyeicosatetraenoic acid ELISA kit

E01A0653 has been referenced in the below publications:

Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.

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