E01L0243 Human Latent membrane protein 1 ELISA kit
The Human Latent membrane protein 1 ELISA kit can be used to identify samples from the human species. Latent membrane protein 1 can also be called LMP 1, LMP-1.
Specifications of Human Latent membrane protein 1 ELISA kit
Product Information | |
Cat. No. | E01L0243 |
Product Name | Human Latent membrane protein 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LMP 1 ELISA kit uses an anti-LMP 1 antibody and an LMP 1-HRP conjugate in a competitive enzyme immunoassay method. LMP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LMP 1 antibody binding site between LMP 1 from samples and LMP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LMP 1. Since the number of sites is limited, as more sites are occupied by LMP 1 from the sample, fewer sites are left to bind LMP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LMP 1 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Latent membrane protein 1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LMP 1 and analogues was observed. | |
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Summary of the Assay Procedure for Human Latent membrane protein 1 ELISA kit
Citations of Human Latent membrane protein 1 ELISA kit
E01L0243 has been referenced in the below publications：
The expression of LMP-1 gene in juve nile lupus nephritis.
