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BlueGene Biotech Human Leukotriene B4 ELISA kit (E01L0037)

E01L0037 Human Leukotriene B4 ELISA kit

The Human Leukotriene B4 ELISA kit can be used to identify samples from the human species. Leukotriene B4 can also be called LTB4, LT-B4.

Products

Specifications of Human Leukotriene B4 ELISA kit

Product Information

Cat. No.

E01L0037

Product Name

Human Leukotriene B4 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LTB4 ELISA kit uses an anti-LTB4 antibody and a LTB4-HRP conjugate in a competitive enzyme immunoassay method. LTB4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LTB4 antibody binding site between LTB4 from samples and LTB4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LTB4. Since the number of sites is limited, as more sites are occupied by LTB4 from the sample, fewer sites are left to bind LTB4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTB4 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Leukotriene B4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LTB4 and analogues was observed.


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Summary of the Assay Procedure for Human Leukotriene B4 ELISA kit

Summary of the Assay Procedure for Human Leukotriene B4 ELISA kit

Citations of Human Leukotriene B4 ELISA kit

E01L0037 has been referenced in the below publications:

Clinical Observation of Concentration Variation of Conleukotriene B4 and Tumornecrosis factor-a During Different Periods of Bronchiectasis and its Relevance with Lung Function.

Effect of Yangyin Yifei Fang on Leukotrience B4 Level in Patients with Chronic Obstructive Pulmonary Disease in Stable Stage of Qi and Yin Deficiency Type.

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