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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Omentin 1 ELISA kit

E01O0022 Human Omentin 1 ELISA kit

The Human Omentin 1 ELISA kit can be used to identify samples from the human species. Omentin 1 can also be called OMT 1, HL1, INTL, ITLN, LFR, HIntL, Omentin, Intelectin 1,Galactofuranose Binding, Intestinal Lactoferrin Receptor,Endothelial lectin HL-1.

Specifications of Human Omentin 1 ELISA kit

Product Information

Cat. No.

E01O0022

Product Name

Human Omentin 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OMT 1 ELISA kit uses an anti-OMT 1 antibody and an OMT 1-HRP conjugate in a competitive enzyme immunoassay method. OMT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OMT 1 antibody binding site between OMT 1 from samples and OMT 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OMT 1. Since the number of sites is limited, as more sites are occupied by OMT 1 from the sample, fewer sites are left to bind OMT 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OMT 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Omentin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OMT 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Omentin 1 ELISA kit

Summary of the Assay Procedure for Human Omentin 1 ELISA kit

Citations of Human Omentin 1 ELISA kit

E01O0022 has been referenced in the below publications:

Omentin-1 is associated with carotid atherosclerosis in patients with metabolic syndrome.

Decreased Levels of Serum Omentin-1 in Patients with Inflammatory Bowel Disease.

To investigate the correlation of Omentin-1 and acute myocardial infarction (AMI).

The correlation of serum omentin-1 levels with the presence of coronary artery disease in patients with type 2 diabetes.

Clinical Analysis of Compound Dingxiangkaiwei Paste Combined Shajiganruji Treatment of 46 Cases of Children with Functional Abdominal Pain.

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