E01M0002 Human α2-Macroglobulin ELISA kit
The Human α2-Macroglobulin ELISA kit can be used to identify samples from the human species. α2-Macroglobulin can also be called α2-MG, A2M, A2MD, CPAMD5, FWP007, S863-7, α2-Macroglobulin, α2M, alpha-2-macroglobulin, transcuprein.
Product Information | |
Cat. No. | E01M0002 |
Product Name | Human α2-Macroglobulin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 µg/ml |
Sensitivity | 0.1 µg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 µg/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 µg/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 µg/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 µg/mL | 1 vial |
STANDARD E (0.5mL) | 10 µg/mL | 1 vial |
STANDARD F (0.5mL) | 25 µg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
α2-MG ELISA kit uses an anti-α2-MG antibody and an α2-MG-HRP conjugate in a competitive enzyme immunoassay method. α2-MG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-α2-MG antibody binding site between α2-MG from samples and α2-MG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of α2-MG. Since the number of sites is limited, as more sites are occupied by α2-MG from the sample, fewer sites are left to bind α2-MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The α2-MG concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between α2-MG and analogues was observed. | |
E01M0002 has been referenced in the below publications：
The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.
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