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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

E01P0183 Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

The Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit can be used to identify samples from the human species. Palate, lung, and nasal epithelium carcinoma associated protein can also be called BPIFA1, SPURT, NASG, LUNX, SPLUNC1, Secretory Protein In Upper Respiratory Tracts, BPI Fold Containing Family A, Member 1, Nasopharyngeal carcinoma-related.

Specifications of Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Product Information

Cat. No.

E01P0183

Product Name

Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PLUNC ELISA kit uses an anti-PLUNC antibody and an PLUNC-HRP conjugate in a competitive enzyme immunoassay method. PLUNC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PLUNC antibody binding site between PLUNC from samples and PLUNC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PLUNC. Since the number of sites is limited, as more sites are occupied by PLUNC from the sample, fewer sites are left to bind PLUNC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLUNC concentration in each sample is interpolated from this standard curve.


Quality Control on Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PLUNC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Summary of the Assay Procedure for Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Citations of Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

E01P0183 has been referenced in the below publications:

To investigate the value of CK, CK7, PLUNC in the diagnosis and treatment of Meningeal Carcinomatosis.

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