E01P0589 Human Platelet activating factor ELISA kit
The Human Platelet activating factor ELISA kit can be used to identify samples from the human species. Platelet activating factor can also be called PAF, PAF-Acether, AGEPC, Acetyl-Glyceryl-Ether-Phosphorylcholine, acetyl-glyceryl-ether-phosphorylcholine.
Product Information | |
Cat. No. | E01P0589 |
Product Name | Human Platelet activating factor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PAF ELISA kit uses an anti-PAF antibody and an PAF-HRP conjugate in a competitive enzyme immunoassay method. PAF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAF antibody binding site between PAF from samples and PAF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAF. Since the number of sites is limited, as more sites are occupied by PAF from the sample, fewer sites are left to bind PAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAF concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PAF and analogues was observed. | |
E01P0589 has been referenced in the below publications：
Effects of Local Mild Hypothermia Therapy On Serum Content of Platelet Activating Factor in Patients with Acute Large Area Cerebral Infarction.
Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation.
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