E01P0029 Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit
The Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit can be used to identify samples from the human species. Procollagen Type Ⅲ N Terminal Propeptide can also be called PⅢNP, P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide.
Product Information | |
Cat. No. | E01P0029 |
Product Name | Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PⅢNP ELISA kit uses an anti-PⅢNP antibody and an PⅢNP-HRP conjugate in a competitive enzyme immunoassay method. PⅢNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PⅢNP antibody binding site between PⅢNP from samples and PⅢNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PⅢNP. Since the number of sites is limited, as more sites are occupied by PⅢNP from the sample, fewer sites are left to bind PⅢNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PⅢNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PⅢNP and analogues was observed. | |
E01P0029 has been referenced in the below publications：
2型糖尿病患者心肌纤维化血清标志物。
Flavonoid-rich Scabiosa comosa inflorescence extract attenuates CCl4-induced hepatic fibrosis by modulating TGF-β-induced Smad3 phosphorylation.
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