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BlueGene Biotech Human Sex Hormone Binding Globulin ELISA kit

E01S0026 Human Sex Hormone Binding Globulin ELISA kit

The Human Sex Hormone Binding Globulin ELISA kit can be used to identify samples from the human species. Sex Hormone Binding Globulincan also be called SHBG, ABP, SBP, TEBG, sex hormone binding globulin, Sex hormone-binding globulin.

Specifications of Human Sex Hormone Binding Globulin ELISA kit

Product Information

Cat. No.

E01S0026

Product Name

Human Sex Hormone Binding Globulin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 nmol/L

Sensitivity

1.0 nmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 nmol/L

1 vial

STANDARD B (0.5mL)

10 nmol/L

1 vial

STANDARD C (0.5mL)

25 nmol/L

1 vial

STANDARD D (0.5mL)

50 nmol/L

1 vial

STANDARD E (0.5mL)

100 nmol/L

1 vial

STANDARD F (0.5mL)

250 nmol/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Sex Hormone Binding Globulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SHBG and analogues was observed.


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Summary of the Assay Procedure for Human Sex Hormone Binding Globulin ELISA kit

Summary of the Assay Procedure for Human Sex Hormone Binding Globulin ELISA kit

Citations of Human Sex Hormone Binding Globulin ELISA kit

E01S0026 has been referenced in the below publications:

Effect of pioglitazone on testosterone in eugonadal men with type 2 diabetes mellitus: a randomized double-blind placebo-controlled study.

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