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BlueGene Biotech Human soluble Cluster of differentiation 40 ligand ELISA kit (E01C0209)

E01C0209 Human soluble Cluster of differentiation 40 ligand ELISA kit

Human soluble Cluster of differentiation 40 ligand ELISA kit is suitable for the detection of samples from human species. Soluble Cluster of differentiation 40 ligand can also be called Scd40l, CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand.

Products

Specifications of Human soluble Cluster of differentiation 40 ligand ELISA kit

Product Information

Cat. No.

E01C0209

Product Name

Human soluble Cluster of differentiation 40 ligand ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Scd40l ELISA kit uses an anti-Scd40l antibody and an Scd40l-HRP conjugate in a competitive enzyme immunoassay method. Scd40l-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Scd40l antibody binding site between Scd40l from samples and Scd40l-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Scd40l. Since the number of sites is limited, as more sites are occupied by Scd40l from the sample, fewer sites are left to bind Scd40l-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Scd40l concentration in each sample is interpolated from this standard curve.


Quality Control on Human soluble Cluster of differentiation 40 ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Scd40l and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human soluble Cluster of differentiation 40 ligand ELISA kit

Summary of the Assay Procedure for Human soluble Cluster of differentiation 40 ligand ELISA kit

Citations of Human soluble Cluster of differentiation 40 ligand ELISA kit

E01C0209 has been referenced in the below publications:

Study on atherosclerotic plaque composition in patients with intermediate coronary stenosis by multidetector computed tomography angiography, serum matrix metalloproteinase 9 and soluble CD40 ligand.

丹红注射液对急性冠状动脉综合征病人血管内皮的保护作用。

The clinical research of serum soluble CD40L and high sensitivie C-reactive protein in patients with acute coronary syndrome.


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