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  • bovine serum albumin elisa 1

BlueGene Biotech Human Osteocalcin ELISA kit

E01O0001 Human Osteocalcin ELISA kit

The Human Osteocalcin ELISA kit can be used to identify samples from the human species. Osteocalcin can also be called Osteocalcin Propeptide, Osteocalcin, Osteocalcin precursor, BGLAP, Bone gamma carboxyglutamate protein, BGP, Bone gamma carboxyglutamate gla protein osteocalcin, Bone Gla protein, Gamma carboxyglutamic acid containing protein.

Specifications of Human Osteocalcin ELISA kit

Product Information

Cat. No.

E01O0001

Product Name

Human Osteocalcin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OC ELISA kit uses an anti-OC antibody and an OC-HRP conjugate in a competitive enzyme immunoassay method. OC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OC antibody binding site between OC from samples and OC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OC. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve.


Quality Control on Human Osteocalcin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Osteocalcin ELISA kit

Summary of the Assay Procedure for Human Osteocalcin ELISA kit

Citations of Human Osteocalcin ELISA kit

E01O0001 has been referenced in the below publications:

Bone plate composed of a ternary nano-hydroxyapatite/polyamide 66/glass fiber composite: biomechanical properties and biocompatibility.

Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities.

Significance of Bone Metabolism Markers in Knee osteoarthritis.

Effects of Salvia Miltiorrhiza on Production of Osteocalcin and Concentration of Calcium Ion in Human Osteoblasts.

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