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BlueGene Biotech Human pregnancy specific glycoprotein 9 ELISA kit

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E01P1062 Human pregnancy specific glycoprotein 9 ELISA kit

The Human pregnancy specific glycoprotein 9 ELISA kit can be used to identify samples from the human species. pregnancy specific glycoprotein 9 can also be called Pregnancy-specific beta-1-glycoprotein 9, PS34, PSBG-11, PSBG-9, PSG11, PSGII, pregnancy specific beta-1-glycoprotein 9.

Specifications of Human pregnancy specific glycoprotein 9 ELISA kit

Product Information

Cat. No.

E01P1062

Product Name

Human pregnancy specific glycoprotein 9 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PSG9 ELISA kit uses an anti-PSG9 antibody and an PSG9-HRP conjugate in a competitive enzyme immunoassay method. PSG9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PSG9 antibody binding site between PSG9 from samples and PSG9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PSG9. Since the number of sites is limited, as more sites are occupied by PSG9 from the sample, fewer sites are left to bind PSG9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSG9 concentration in each sample is interpolated from this standard curve.


Quality Control on Human pregnancy specific glycoprotein 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PSG9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human pregnancy specific glycoprotein 9 ELISA kit

Summary of the Assay Procedure for Human pregnancy specific glycoprotein 9 ELISA kit

Citations of Human pregnancy specific glycoprotein 9 ELISA kit

E01P1062 has been referenced in the below publications:

Post-surgical resection prognostic value of combined OPN, MMP7, and PSG9 plasma biomarkers in hepatocellular carcinoma.

应用分泌/释放蛋白质组学鉴定HBV相关的肝细胞癌血浆标志物.

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