E01P1062 Human pregnancy specific glycoprotein 9 ELISA kit
The Human pregnancy specific glycoprotein 9 ELISA kit can be used to identify samples from the human species. pregnancy specific glycoprotein 9 can also be called Pregnancy-specific beta-1-glycoprotein 9, PS34, PSBG-11, PSBG-9, PSG11, PSGII, pregnancy specific beta-1-glycoprotein 9.
Product Information | |
Cat. No. | E01P1062 |
Product Name | Human pregnancy specific glycoprotein 9 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PSG9 ELISA kit uses an anti-PSG9 antibody and an PSG9-HRP conjugate in a competitive enzyme immunoassay method. PSG9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PSG9 antibody binding site between PSG9 from samples and PSG9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PSG9. Since the number of sites is limited, as more sites are occupied by PSG9 from the sample, fewer sites are left to bind PSG9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSG9 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PSG9 and analogues was observed. | |
E01P1062 has been referenced in the below publications：
Post-surgical resection prognostic value of combined OPN, MMP7, and PSG9 plasma biomarkers in hepatocellular carcinoma.
应用分泌/释放蛋白质组学鉴定HBV相关的肝细胞癌血浆标志物.
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