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BlueGene Biotech Human Renin ELISA kit

E01R0022 Human Renin ELISA kit

The Human Renin ELISA kit can be used to identify samples from the human species. Renin can also be called Renin, REN, HNFJ2, renin, ADTKD4, RTD, Angiotensinogenase.

Specifications of Human Renin ELISA kit

Product Information

Cat. No.

E01R0022

Product Name

Human Renin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Renin ELISA kit uses an anti-Renin antibody and a Renin-HRP conjugate in a competitive enzyme immunoassay method. Renin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Renin antibody binding site between Renin from samples and Renin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Renin. Since the number of sites is limited, as more sites are occupied by Renin from the sample, fewer sites are left to bind Renin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Renin concentration in each sample is interpolated from this standard curve.


Quality Control on Human Renin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Renin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Renin ELISA kit

Summary of the Assay Procedure for Human Renin ELISA kit

Citations of Human Renin ELISA kit

E01R0022 has been referenced in the below publications:

Changes of ET-1 and NO in the maintenance hemodialysis complicated with intradialytic hypertension.

Effects of hemodialysis on the cardiovascular function in patients with intradialytic hypertension and the related mechanism.

Changes of the Renin-angiotensin-aldosterone Sysetem During the Perioperative Period in Patients with Mitral Valve Replacement its Clinical Significance. 

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