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BlueGene Biotech Human Vascular Endothelial Growth Factor ELISA kit

E01V0010 Human Vascular Endothelial Growth Factor ELISA kit

The Human Vascular Endothelial Growth Factor ELISA kit can be used to identify samples from the human species. Vascular Endothelial Growth Factor can also be called VEGF, Vascuoar endothelial growth factor A, VEGF A, vascular endothelial growth factor A isoform 2 precursor, Vegf, VEGFA,  MVCD1, VEGF, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF A Precursor.

Specifications of Human Vascular Endothelial Growth Factor ELISA kit

Product Information

Cat. No.

E01V0010

Product Name

Human Vascular Endothelial Growth Factor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VEGF ELISA kit uses an anti-VEGF antibody and an VEGF-HRP conjugate in a competitive enzyme immunoassay method. VEGF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VEGF antibody binding site between VEGF from samples and VEGF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VEGF. Since the number of sites is limited, as more sites are occupied by VEGF from the sample, fewer sites are left to bind VEGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Vascular Endothelial Growth Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VEGF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Vascular Endothelial Growth Factor ELISA kit

Summary of the Assay Procedure for Human Vascular Endothelial Growth Factor ELISA kit

Citations of Human Vascular Endothelial Growth Factor ELISA kit

E01V0010 has been referenced in the below publications:

Emodin-Loaded Magnesium Silicate Hollow Nanocarriers for Anti-Angiogenesis Treatment through Inhibiting VEGF.

The relationship between circulating tissue transglutaminase, soluble fms-like tyrosine kinase-1, soluble endoglin and vascular endothelial growth factor in pre-eclampsia.

Comparison of the Effectiveness of High-Intensity Interval Training in Hypoxia and Normoxia in Healthy Male Volunteers:A Pilot Study.

The role and mechanism of asymmetric dimethylarginine in fetal growth restriction via interference with endothelial function and angiogenesis.

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