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BlueGene Biotech Human soluble endothelial protein C receptor ELISA kit

E01S0249 Human soluble endothelial protein C receptor ELISA kit

The Human soluble endothelial protein C receptor ELISA kit can be used to identify samples from the human species. Soluble endothelial protein C receptor can also be called sEPCR, SPROCR, SCCCA, SCCD41, SEPCR, sprotein C receptor.

Specifications of Human soluble endothelial protein C receptor ELISA kit

Product Information

Cat. No.

E01S0249 

Product Name

Human soluble endothelial protein C receptor ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SEPCR ELISA kit uses an anti-SEPCR antibody and an SEPCR-HRP conjugate in a competitive enzyme immunoassay method. SEPCR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SEPCR antibody binding site between SEPCR from samples and SEPCR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SEPCR. Since the number of sites is limited, as more sites are occupied by SEPCR from the sample, fewer sites are left to bind SEPCR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEPCR concentration in each sample is interpolated from this standard curve.


Quality Control on Human soluble endothelial protein C receptor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SEPCR and analogues was observed.


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Summary of the Assay Procedure for Human soluble endothelial protein C receptor ELISA kit

Summary of the Assay Procedure for Human soluble endothelial protein C receptor ELISA kit

Citations of Human soluble endothelial protein C receptor ELISA kit

E01S0249 has been referenced in the below publications:

Carbon monoxide-releasing molecule-2 suppresses thrombomodulin and endothelial protein C receptor expression of human umbilical vein endothelial cells induced by lipopolysaccharide in vitro.

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