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BlueGene Biotech Human Total prostate specific antigen ELISA kit (E01T0533)

E01T0533 Human Total prostate specific antigen ELISA kit

The Human Total prostate s pecific antigen ELISA kit can be used to identify samples from the human species. Total prostate s pecific antigen can also be called TPSA, fPSA, APS, KLK2A1, hK3, Kallikrein-3, Kallikrein-Related Peptidase 3, Gamma-seminoprotein.

Products

Specifications of Human Total prostate specific antigen ELISA kit

Product Information

Cat. No.

E01T0533

Product Name

Human Total prostate specific antigen ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TPSA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TPSA. Standards or samples are then added to the microtiter plate wells and TPSA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TPSA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TPSA are added to each well to “sandwich” the TPSA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TPSA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPSA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Total prostate specific antigen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TPSA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Total prostate specific antigen ELISA kit

Summary of the Assay Procedure for Human Total prostate specific antigen ELISA kit

Citations of Human Total prostate specific antigen ELISA kit

E01T0533 has been referenced in the below publications:

Association of prostate specific antigen concentration with (klk3) rs2735839 in infertile patients.

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