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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Activated Protein C ELISA kit

E03A0842 Mouse Activated Protein C ELISA kit

The Mouse Activated Protein C ELISA kit can be used to identify samples from the mouse species. Activated Protein C can also be called PROC, APC, PC, PROC1, THPH3, THPH4, protein C, inactivator of coagulation factors Va and VIIIa.

Specifications of Mouse Activated Protein C ELISA kit

Product Information

Cat. No.

E03A0842

Product Name

Mouse Activated Protein C ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500pg/mL

Sensitivity

1.0pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

25 pg/ml

1 vial

STANDARD C (0.5mL)

50 pg/ml

1 vial

STANDARD D (0.5mL)

100 pg/ml

1 vial

STANDARD E (0.5mL)

250 pg/ml

1 vial

STANDARD F (0.5mL)

500 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

APC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APC antibody and an APC-HRP conjugate. The assay sample and buffer are incubated together with APC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APC concentration since APC from samples and APC-HRP conjugate compete for the anti-APC antibody binding site. Since the number of sites is limited, as more sites are occupied by APC from the sample, fewer sites are left to bind APC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APC concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Activated Protein C ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between APC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Activated Protein C ELISA kit

Summary of the Assay Procedure for Mouse Activated Protein C ELISA kit

Citations of Mouse Activated Protein C ELISA kit

E03A0842 has been referenced in the below publications:

Enhanced induction of heme oxygenase-1 suppresses thrombus formation and affects the protein C system in sepsis.




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