E03H0211 Mouse High Density Lipoprotein ELISA kit
The Mouse High Density Lipoprotein ELISA kit can be used to identify samples from the mouse species. High Density Lipoprotein can also be called HDL, Apo-AI, APOA1, APOA2, APOC1.
Specifications of Mouse High Density Lipoprotein ELISA kit
Product Information | |
Cat. No. | E03H0211 |
Product Name | Mouse High Density Lipoprotein ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 25 ng/ml | 1 vial |
STANDARD C (0.5mL) | 50 ng/ml | 1 vial |
STANDARD D (0.5mL) | 100 ng/ml | 1 vial |
STANDARD E (0.5mL) | 250 ng/ml | 1 vial |
STANDARD F (0.5mL) | 500 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HDL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HDL antibody and an HDL-HRP conjugate. The assay sample and buffer are incubated together with HDL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HDL concentration since HDL from samples and HDL-HRP conjugate compete for the anti-HDL antibody binding site. Since the number of sites is limited, as more sites are occupied by HDL from the sample, fewer sites are left to bind HDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HDL concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse High Density Lipoprotein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HDL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse High Density Lipoprotein ELISA kit
Citations of Mouse High Density Lipoprotein ELISA kit
E03H0211 has been referenced in the below publications：
Effects of medium-chain fatty acids on cholesterol metabolism and their mechanisms in mice.
种具有降血脂降血糖活性的组合物及其制备方法与应用。
