E03H0100 Mouse Heparanase ELISA kit
The Mouse Heparanase ELISA kit can be used to identify samples from the mouse species. Heparanase can also be called HPSE, HPA, HPA1, HPR1, HPSE1, HSE1, heparanase.
Specifications of Mouse Heparanase ELISA kit
Product Information | |
Cat. No. | E03H0100 |
Product Name | Mouse Heparanase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HPA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HPA antibody and an HPA-HRP conjugate. The assay sample and buffer are incubated together with HPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPA concentration since HPA from samples and HPA-HRP conjugate compete for the anti-HPA antibody binding site. Since the number of sites is limited, as more sites are occupied by HPA from the sample, fewer sites are left to bind HPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Heparanase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HPA and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Heparanase ELISA kit
Citations of Mouse Heparanase ELISA kit
E03H0100 has been referenced in the below publications：
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Effect of medium chain triglycerides on brown adipose tissue in obese mice.
Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet.
