E03I0058 Mouse Immunoglobulin G ELISA kit
The Mouse Immunoglobulin G ELISA kit can be used to identify samples from the mouse species. Immunoglobulin G can also be called Immunoglobulin G, IgG.
Specifications of Mouse Immunoglobulin G ELISA
Product Information | |
Cat. No. | E03I0058 |
Product Name | Mouse Immunoglobulin G ELISA |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ug/ml |
Sensitivity | 1.0ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 50 ug/ml | 1 vial |
STANDARD C (0.5mL) | 100 ug/ml | 1 vial |
STANDARD D (0.5mL) | 250 ug/ml | 1 vial |
STANDARD E (0.5mL) | 500 ug/ml | 1 vial |
STANDARD F (0.5mL) | 1000 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgG antibody and an IgG-HRP conjugate. The assay sample and buffer are incubated together with IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG concentration since IgG from samples and IgG-HRP conjugate compete for the anti-IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG from the sample, fewer sites are left to bind IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Immunoglobulin G ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IgG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Immunoglobulin G ELISA kit
Citations of Mouse Immunoglobulin G ELISA kit
E03I0058 has been referenced in the below publications：
Influence on gastric mucosa specific antibody in newborn mice after oral immunization with inactived H. pylori.
The effect of langchuangqing granule on anti-ds-DNA antibody，IgG and IgA level in MRL/lpr lupus mice.
