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BlueGene Biotech Mouse Immunoglobulin G ELISA kit (E03I0058)

E03I0058 Mouse Immunoglobulin G ELISA kit

The Mouse Immunoglobulin G ELISA kit can be used to identify samples from the mouse species. Immunoglobulin G can also be called Immunoglobulin G, IgG.

Products

Specifications of Mouse Immunoglobulin G ELISA

Product Information

Cat. No.

E03I0058

Product Name

Mouse Immunoglobulin G ELISA 

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

50 ug/ml

1 vial

STANDARD C (0.5mL)

100 ug/ml

1 vial

STANDARD D (0.5mL)

250 ug/ml

1 vial

STANDARD E (0.5mL)

500 ug/ml

1 vial

STANDARD F (0.5mL)

1000 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgG antibody and an IgG-HRP conjugate. The assay sample and buffer are incubated together with IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG concentration since IgG from samples and IgG-HRP conjugate compete for the anti-IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG from the sample, fewer sites are left to bind IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Immunoglobulin G ELISA kit

Summary of the Assay Procedure for Mouse Immunoglobulin G ELISA kit

Citations of Mouse Immunoglobulin G ELISA kit

E03I0058 has been referenced in the below publications:

Influence on gastric mucosa specific antibody in newborn mice after oral immunization with inactived H. pylori.

The effect of langchuangqing granule on anti-ds-DNA antibody,IgG and IgA level in MRL/lpr lupus mice.


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