E03I0308 Mouse Interleukin 2 ELISA kit
The Mouse Interleukin 2 ELISA kit can be used to identify samples from the mouse species. Interleukin 2 can also be called IL2, Interleukin-2, IL-2, T-cell growth factor, Aldesleukin, Lymphokine, POIL2, T Cell Growth Factor, TCGF.
Product Information | |
Cat. No. | E03I0308 |
Product Name | Mouse Interleukin 2 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 50 pg/ml | 1 vial |
STANDARD C (0.5mL) | 100 pg/ml | 1 vial |
STANDARD D (0.5mL) | 250 pg/ml | 1 vial |
STANDARD E (0.5mL) | 500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 1000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL2 antibody and an IL2-HRP conjugate. The assay sample and buffer are incubated together with IL2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL2 concentration since IL2 from samples and IL2-HRP conjugate compete for the anti-IL2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL2 from the sample, fewer sites are left to bind IL2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL2 and analogues was observed. | |
E03I0308 has been referenced in the below publications：
Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.
Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.
The study of Sagittaria sagittifolia polysaccharide about its regulating immune function on mice and its possible mechanism on mTOR signaling pathway.
THE PRELIMINARILYSTUDYOFANTI-TUMOR EFFECT AND ITS MOLECULAR MECHANISMS OF CALCULUS BOVIS CULTIVATED BY GLUCURONIDASE.
Different kinds of moxa smoke effects on immune function in mice.
Warming moxibustion increases serum levels of interleukin-2 and transforming growth factor beta in ulcerative colitis mice.
THE PRELIMINARILY STUDY OF ANTI-TUMOR EFFECT AND ITS MOLECULAR MECHANISMS OF CALCULUS BOVIS CULTIVATED BY GLUCURONIDASE.
Enhancement effect of Sagittaria sagittifolia polysaccharide on immune function of macrophages through mTOＲ signal pathway.
Study of Regulating Effect of Seriphidium Decoction on Immunologic Function of Normal Mice.
Exosomes derived from Rab27a‑overexpressing tumor cells elicit efficient induction of antitumor immunity.
Ultrasound‑targeted microbubble destruction‑mediated Foxp3 knockdown may suppress the tumor growth of HCC mice by relieving immunosuppressive Tregs function.
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