En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse E2 Estradiol ELISA kit

E03E0023 Mouse E2 Estradiol ELISA kit

The Mouse E2 Estradiol ELISA kit can be used to identify samples from the mouse species. E2 Estradiol can also be called  Estradiol, E2 estradiol, 17B-Estradiol, E2, Oestradiol, Beta-Estradiol.

Specifications of Mouse E2 Estradiol ELISA kit

Product Information

Cat. No.

E03E0023

Product Name

Mouse E2 Estradiol ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse E2 Estradiol ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between E2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse E2 Estradiol ELISA kit

Summary of the Assay Procedure for Mouse E2 Estradiol ELISA kit

Citations of Mouse E2 Estradiol ELISA kit

E03E0023 has been referenced in the below publications:

Effect of follicle stimulating hormone on cryopreserved sheep ovarian tissue xenotransplantation.

Effects of carbon disulfide exposure during peri-implantation onestrogen receptor-α expression in uterus and serum level of estrogen in pregnant mice.

Different kinds of moxa smoke effects on physical of the pregnant mice and the growth and development of the fetal mice.

Down-regulation of uterine LIF expression induced by the hormonal level disorder causes embryo implantation loss after mice exposed to carbon disulfide at peri-implantation.

Inhibitory activities of some traditional Chinese herbs against testosterone 5α-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice.

Long non‑coding RNA NEAT1 promotes mouse granulosa cell proliferation and estradiol synthesis by sponging miR‑874‑3p.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.