Mouse Matrix Metalloproteinase 2 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Matrix Metalloproteinase 2 ELISA kit

E03M0304 Mouse Matrix Metalloproteinase 2 ELISA kit

The Mouse Matrix Metalloproteinase 2 ELISA kit can be used to identify samples from the mouse species. Matrix Metalloproteinase 2 can also be called MMP 2, 72 kDa gelatinase, 72kD type IV collagenase, CLG 4, CLG 4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil, Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase, Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), Matrix Metalloproteinase 2, Matrix metalloproteinase II, MMP II, MONA, Neutrophil gelatinase, TBE 1．

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Specifications of Mouse Matrix Metalloproteinase 2 ELISA kit

Product Information
Cat. No.	E03M0304
Product Name	Mouse Matrix Metalloproteinase 2 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	0.5 ng/ml	1 vial
STANDARD C (0.5mL)	1.0 ng/ml	1 vial
STANDARD D (0.5mL)	2.5 ng/ml	1 vial
STANDARD E (0.5mL)	5.0ng/ml	1 vial
STANDARD F (0.5mL)	10 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

MMP 2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP 2 antibody and a MMP 2-HRP conjugate. The assay sample and buffer are incubated together with MMP 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP 2 concentration since MMP 2 from samples and MMP 2-HRP conjugate compete for the anti-MMP 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP 2 from the sample, fewer sites are left to bind MMP 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 2 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Matrix Metalloproteinase 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between MMP 2 and analogues was observed.