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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Interleukin 4 ELISA kit

E03I0007 Mouse Interleukin 4 ELISA kit

The Mouse Interleukin 4 ELISA kit can be used to identify samples from the mouse species. Interleukin 4 can also be called IL4, BSF1, BCGF1, B Cell Stimulatory Factor 1, Lymphocyte stimulatory factor 1, B Cell Growth Factor, Binetrakin, Pitrakinra.

Specifications of Mouse Interleukin 4 ELISA kit

Product Information

Cat. No.

E03I0007

Product Name

Mouse Interleukin 4 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL4 antibody and an IL4-HRP conjugate. The assay sample and buffer are incubated together with IL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL4 concentration since IL4 from samples and IL4-HRP conjugate compete for the anti-IL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL4 from the sample, fewer sites are left to bind IL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL4 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Interleukin 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interleukin 4 ELISA kit

Summary of the Assay Procedure for Mouse Interleukin 4 ELISA kit

Citations of Mouse Interleukin 4 ELISA kit

E03I0007 has been referenced in the below publications:

Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.

Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.

Study on macrophage polarization and cellular immune response against fasciola gigantic and its excretory-secretory products.

Anti-asthmatic effect of Xuebijing and potential role of heme oxygenase-1 in an ovalbumin-induced murine allergic rhinitis model .

The therapeutic effects of UTI on Allergic rhinitis and the relationship with heme oxygenase-l.

乌司他丁对支气管哮喘小鼠氧化应激及血红素加氧酶1的影响。

消疹止痒喷剂对皮炎及瘙痒模型的药效学观察。


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