E03P0661 Mouse Protein Kinase B ELISA kit
The Mouse Protein Kinase B ELISA kit can be used to identify samples from the mouse species. Protein Kinase B can also be called Akt, PIKBa.
Mouse Protein Kinase B ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
|0 ng/ml||1 vial|
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
PIKBa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PIKBa antibody and an PIKBa-HRP conjugate. The assay sample and buffer are incubated together with PIKBa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIKBa concentration since PIKBa from samples and PIKBa-HRP conjugate compete for the anti-PIKBa antibody binding site. Since the number of sites is limited, as more sites are occupied by PIKBa from the sample, fewer sites are left to bind PIKBa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIKBa concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between PIKBa and analogues was observed.
E03P0661 has been referenced in the below publications：
Clinical Evaluation of NB-UVB Combined with Tuibai Decoction for Treating Vitiligo Vulgaris in 59 Cases.
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