E03P0648 Mouse Prostaglandin H2 ELISA kit
The Mouse Prostaglandin H2 ELISA kit can be used to identify samples from the mouse species. Prostaglandin H2 can also be called PG-H2, PGH2.
Specifications of Mouse Prostaglandin H2 ELISA kit
Product Information | |
Cat. No. | E03P0648 |
Product Name | Mouse Prostaglandin H2 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 25 ng/ml | 1 vial |
STANDARD C (0.5mL) | 50 ng/ml | 1 vial |
STANDARD D (0.5mL) | 100 ng/ml | 1 vial |
STANDARD E (0.5mL) | 250 ng/ml | 1 vial |
STANDARD F (0.5mL) | 500 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PGH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PGH2 antibody and an PGH2-HRP conjugate. The assay sample and buffer are incubated together with PGH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGH2 concentration since PGH2 from samples and PGH2-HRP conjugate compete for the anti-PGH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGH2 from the sample, fewer sites are left to bind PGH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGH2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Prostaglandin H2 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PGH2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Prostaglandin H2 ELISA kit
Citations of Mouse Prostaglandin H2 ELISA kit
E03P0648 has been referenced in the below publications：
Reduced prostaglandin I signaling in Arid5b primary skeletal muscle cells attenuates myogenesis.
Reduced prostaglandin I2 signaling in Arid5b2/2primary skeletal muscle cells attenuates myogenesis.
