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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Prostaglandin H2 ELISA kit

E03P0648 Mouse Prostaglandin H2 ELISA kit

The Mouse Prostaglandin H2 ELISA kit can be used to identify samples from the mouse species. Prostaglandin H2 can also be called PG-H2, PGH2.

Specifications of Mouse Prostaglandin H2 ELISA kit

Product Information

Cat. No.

E03P0648

Product Name

Mouse Prostaglandin H2 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500ng/mL

Sensitivity

1.0ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

25 ng/ml

1 vial

STANDARD C (0.5mL)

50 ng/ml

1 vial

STANDARD D (0.5mL)

100 ng/ml

1 vial

STANDARD E (0.5mL)

250 ng/ml

1 vial

STANDARD F (0.5mL)

500 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PGH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PGH2 antibody and an PGH2-HRP conjugate. The assay sample and buffer are incubated together with PGH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGH2 concentration since PGH2 from samples and PGH2-HRP conjugate compete for the anti-PGH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGH2 from the sample, fewer sites are left to bind PGH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGH2 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Prostaglandin H2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PGH2 and analogues was observed.


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Summary of the Assay Procedure for Mouse Prostaglandin H2 ELISA kit

Summary of the Assay Procedure for Mouse Prostaglandin H2 ELISA kit

Citations of Mouse Prostaglandin H2 ELISA kit

E03P0648 has been referenced in the below publications:

Reduced prostaglandin I signaling in Arid5b primary skeletal muscle cells attenuates myogenesis.

Reduced prostaglandin I2 signaling in Arid5b2/2primary skeletal muscle cells attenuates myogenesis.

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