E03T0130 Mouse Transferrin ELISA kit
The Mouse Transferrin ELISA kit can be used to identify samples from the mouse species. Transferrin can also be called TF, Siderophilin, Serotransferrin, or Beta-1 metal-binding globulin.
Specifications of Mouse Transferrin ELISA kit
Product Information | |
Cat. No. | E03T0130 |
Product Name | Mouse Transferrin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TF antibody and an TF-HRP conjugate. The assay sample and buffer are incubated together with TF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TF concentration since TF from samples and TF-HRP conjugate compete for the anti-TF antibody binding site. Since the number of sites is limited, as more sites are occupied by TF from the sample, fewer sites are left to bind TF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TF concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Transferrin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TF and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Transferrin ELISA kit
Citations of Mouse Transferrin ELISA kit
E03T0130 has been referenced in the below publications：
Effect on the Expression of Macrophages Transferrin Receptor and Ferritin in Macrophages by Mycobacterium Tuberculosis.
Expression of TfR and Lf in Macrophages Infected with Different Virulence Mycobacterium Tuberculosis.
