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BlueGene Biotech Human Interleukin 1β ELISA kit (NE01I0010)

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NE01I0010 Human Interleukin 1β ELISA kit

Human Interleukin 1β ELISA kit is suitable for the detection of samples from human species. Interleukin 1β can also be called IL 1β, IL-1B, Interleukin-1 beta, IL 1, IL 1 beta.

Products

Specifications of Human Interleukin 1β ELISA kit

Product Information

Cat. NO.

NE01I0010

Products Name

Human Interleukin 1β ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

15.63-1000 pg/ml

Sensitivity

2.6pg/ml

Principal

Sandwich ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, and tissue homogenates

Assay Time

3 hours and 45 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

Reagents

Specification

Quantity

Pre-Coated Microplate (Detachable)

96 wells

1 plate (Keep Sealed)

Standard (Stock Solution, 10ng/ml)

100ul

1 vial

Standard Diluent S1

950ul

1 vial

Standard Diluent S2

500ul

1 vial

Standard Diluent S3

500ul

1 vial

Standard Diluent S4

500ul

1 vial

Standard Diluent S5

500ul

1 vial

Standard Diluent S6

500ul

1 vial

Standard Diluent S7

500ul

1 vial

Standard Diluent S8500ul1 vial

Detection antibody(30×)

400ul

1 vial

Detection antibody Diluent

10.5ml

1 vial

HRP-Streptavidin (150×)

120ul

1 vial

HRP-Streptavidin Diluent

10.5ml

1 vial

TMB Substrates

11ml

1 vial (Avoid Light)

Stop Solution

6ml

1 vial

Wash Solution (100×)

10ml

1 vial

Plate Sealer


4 pieces

Instruction Manual


1


Principle of the Assay

IL-1β ELISA kit applies the solid phase sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with an antibody specific to IL-1β. Standards or samples are then added to the microtiter plate wells and IL-1βif present, will bind to the antibody pre-coatedwells.Then a biotin-conjugateddetection antibody specific to IL-1βis added to the wells to form a pre-coatedantibody-antigen-detection antibody Complex. After washing, Streptavidinconjugated Horseradish Peroxidase (HRP)is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns to blue after oxidating by HRP, and finally turns to yellow after adding the stop solution. Color develops in proportion to the amount of IL-1βbound in theinitial steps. The color is measured spectrophotometrically at a wavelength of 450nm. The concentration of IL-1βin the samples is then determined by comparing the O.D. of the samples to the standard curve.


Quality Control on Human Interleukin 1β ELISA kit

Coefficient of Variance

Intra Variation: 4.65-8.15%

Inter Variation: 1.09-6.20%

Recovery

80-111% (serum)

Linearity

Diluent Ratio

Range %

1:02

100.44-106.25

1:04

94.45-99.72

1:08

99.62-102.77

1:16

90.70-105.58

1:32

106.01-120.38

Specificity/

Cross-reactivity

Sample

Cross-Reactivity(%)

IL-6

---

CCL3

---

Mouse serum

---

Bovine serum

---


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Interleukin 1β ELISA kit

Summary of the Assay Procedure for Human Interleukin 1β ELISA kit

Citations of Human Interleukin 1β ELISA kit

Not available

Product: Not available

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