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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Acetylcholine ELISA kit (E02A0711)

E02A0711 Rat Acetylcholine ELISA kit

The Rat Acetylcholine ELISA kit can be used to identify samples from the rat species. Acetylcholine can also be called Acetylcholine,ACh, acetycholine, ACH.

Products

Specifications of Rat Acetylcholine ELISA kit

Product Information

Cat. No.

E02A0711

Product Name

Rat Acetylcholine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACH ELISA kit uses an anti-ACH antibody and an ACH-HRP conjugate in a competitive enzyme immunoassay method. ACH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACH antibody binding site between ACH from samples and ACH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACH. Since the number of sites is limited, as more sites are occupied by ACH from the sample, fewer sites are left to bind ACH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACH concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Acetylcholine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Acetylcholine ELISA kit

Summary of the Assay Procedure for Rat Acetylcholine ELISA kit

Citations of Rat Acetylcholine ELISA kit

E02A0711 has been referenced in the below publications:

Alteration in amyloid β42, phosphorylated tau protein, interleukin 6, and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.

Effect of Electro-acupuncture on Contents of Serum Aeuropeptide and Acetylcholine and Blood Pressure in Stress-induced PHT Rats.

Alteration in amyloid β42,phosphorylated tau protein, interleukin 6,and acetylcholine during diabetes-accelerated memory dysfunction in diabetic rats: correlation of amyloid β42 with changes in glucose metabolism.


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