E02A0842 Rat Activated protein C ELISA kit
The Rat Activated protein C ELISA kit can be used to identify samples from the rat species. Activated protein C can also be called PROC, APC, PC, PROC1, THPH3, THPH4, protein C, inactivator of coagulation factors Va and VIIIa.
Product Information | |
Cat. No. | E02A0842 |
Product Name | Rat Activated protein C ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
APC ELISA kit uses an anti-APC antibody and an APC-HRP conjugate in a competitive enzyme immunoassay method. APC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-APC antibody binding site between APC from samples and APC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of APC. Since the number of sites is limited, as more sites are occupied by APC from the sample, fewer sites are left to bind APC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APC concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between APC and analogues was observed. | |
E02A0842 has been referenced in the below publications：
Effects of heme oxygenase-1 on microcirculation and hepatic function in aged septic rats NAN Chuan-Chuan，MENG Xiang-Lin，FEI Dong-Sheng，et al. The First Affiliated Hospital of Harbin Medical University，Harbin 150001，Heilongjiang，China.
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