En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Atrial Natriuretic Peptide ELISA kit

E02A0493 Rat Atrial Natriuretic Peptide ELISA kit

The Rat Atrial Natriuretic Peptide ELISA kit can be used to identify samples from the rat species. Atrial Natriuretic Peptide can also be called NPPA, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, Atrial natriuretic peptide, natriuretic peptide A.

Specifications of Rat Atrial Natriuretic Peptide ELISA kit

Product Information

Cat. No.

E02A0493

Product Name

Rat Atrial Natriuretic Peptide ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ANP ELISA kit uses an anti-ANP antibody and an ANP-HRP conjugate in a competitive enzyme immunoassay method. ANP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ANP antibody binding site between ANP from samples and ANP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ANP. Since the number of sites is limited, as more sites are occupied by ANP from the sample, fewer sites are left to bind ANP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Atrial Natriuretic Peptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ANP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Atrial Natriuretic Peptide ELISA kit

Summary of the Assay Procedure for Rat Atrial Natriuretic Peptide ELISA kit

Citations of Rat Atrial Natriuretic Peptide ELISA kit

E02A0493 has been referenced in the below publications:

2-Deoxy-D-Glucose (2-DG) Induced Cardiac Toxicity in Rat: NTproBNP and BNP as Potential Early Cardiac Safety Biomarkers.

Effect of acupuncture on the expression of ANP, ACE2 and Ang (1-7)/ AngⅡ ratio in heart tissue of spontaneously hypertensive rats.

The Falconoid Luteolin Mitigates the Myocardial Inflammatory Response Induced by High-Carbohydrate/High-Fat Diet in Wistar Rats.

Yogurt Containing the Probacteria Lactobacillus acidophilus Combined with Natural Antioxidants Mitigates Doxorubicin-Induced Cardiomyopathy in Rats.

Sacubitril/Valsartan Decreases Atrial Fibrillation Susceptibility by Inhibiting Angiotensin II-Induced Atrial Fibrosis Through p-Smad2/3, p-JNK, and p-p38 Signaling Pathways.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.