E02A1970 Rat Antithrombin III ELISA kit
The Rat Antithrombin III ELISA kit can be used to identify samples from the rat species. Antithrombin III can also be called SERPINC1, AT3, AT3D, ATIII, THPH7, serpin family C member 1, ATIII-R2, ATIII-T2, ATIII-T1, AT III.
Product Information | |
Cat. No. | E02A1970 |
Product Name | Rat Antithrombin III ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ug/mL |
Sensitivity | 1.0 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 50 ug/mL | 1 vial |
STANDARD C (0.5mL) | 100 ug/mL | 1 vial |
STANDARD D (0.5mL) | 250 ug/mL | 1 vial |
STANDARD E (0.5mL) | 500 ug/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
pSERPINC1 ELISA kit uses an anti-IpSERPINC1 antibody and an IpSERPINC1-HRP conjugate in a competitive enzyme immunoassay method. IpSERPINC1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IpSERPINC1 antibody binding site between IpSERPINC1 from samples and IpSERPINC1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IpSERPINC1. Since the number of sites is limited, as more sites are occupied by IpSERPINC1 from the sample, fewer sites are left to bind IpSERPINC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IpSERPINC1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SERPINC1 and analogues was observed. | |
E02A1970 has been referenced in the below publications：
Study on Mechanism of Yiqi Huoxue Compound Anti-arterial Thrombosis and Its Action.
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