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BlueGene Biotech Rat Soluble interleukin 6 receptor ELISA kit (E02S0260)

E02S0260 Rat Soluble interleukin 6 receptor ELISA kit

The Rat Soluble interleukin 6 receptor ELISA kit can be used to identify samples from the rat species. Soluble interleukin 6 receptor can also be called CD126, gp80, IL6R, IL-6R-1, IL-6R-Alpha, IL6RA, IL-6 receptor subunit alpha, Membrane glycoprotein 80.

Products

Specifications of Rat Soluble interleukin 6 receptor ELISA kit

Product Information

Cat. No.

E02S0260

Product Name

Rat Soluble interleukin 6 receptor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Sil 6R/cd126 ELISA kit uses an anti-Sil 6R/cd126 antibody and an Sil 6R/cd126-HRP conjugate in a competitive enzyme immunoassay method. Sil 6R/cd126-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Sil 6R/cd126 antibody binding site between Sil 6R/cd126 from samples and Sil 6R/cd126-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Sil 6R/cd126. Since the number of sites is limited, as more sites are occupied by Sil 6R/cd126 from the sample, fewer sites are left to bind Sil 6R/cd126-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Sil 6R/cd126 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Soluble interleukin 6 receptor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Sil 6R/cd126 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Soluble interleukin 6 receptor ELISA kit

Summary of the Assay Procedure for Rat Soluble interleukin 6 receptor ELISA kit

Citations of Rat Soluble interleukin 6 receptor ELISA kit

E02S0260 has been referenced in the below publications:

Effect of Electroacupuncture on the Expressions of IL-6 and sIL-6R during Different Periods of Model Rats with Chronic Restraint Stress.


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