E02B0138 Rat Bone Alkaline Phosphatase ELISA kit
The Rat Bone Alkaline Phosphatase ELISA kit can be used to identify samples from the rat species. Bone Alkaline Phosphatase can also be called BALP; AKP2; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; APTNAP; BAP.
Product Information | |
Cat. No. | E02B0138 |
Product Name | Rat Bone Alkaline Phosphatase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BALP ELISA kit uses an anti-BALP antibody and an BALP-HRP conjugate in a competitive enzyme immunoassay method. BALP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-BALP antibody binding site between BALP from samples and BALP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of BALP. Since the number of sites is limited, as more sites are occupied by BALP from the sample, fewer sites are left to bind BALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BALP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between BALP and analogues was observed. | |
E02B0138 has been referenced in the below publications：
Experimental Study of Herba Epimedii and Fructus Ligustri Lucid on the Glucocorticoid-induced Osteoporosis Rats based on regulation of TGF-beta 1/Smads signaling pathway.
麻黄、五味子配伍地塞米松对肺纤维化大鼠代谢差异性研究。
Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats.
Effects of Aluminum Exposure on the Bone Stimulatory Growth Factors in Rats.
The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolism.
Effects of the combined Herba Epimedii and Fructus Ligustri Lucidi on bone turnover and TGF-β1/Smads pathway in GIOP rats.
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