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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cyclic Guanosine Monophosphate ELISA kit

E02G0003 Rat Cyclic Guanosine Monophosphate ELISA kit

The Rat Cyclic Guanosine Monophosphate ELISA kit can be used to identify samples from the rat species. Cyclic Guanosine Monophosphate can also be called Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate, cGMP.

Specifications of Rat Cyclic Guanosine Monophosphate ELISA kit

Product Information

Cat. No.

E02G0003

Product Name

Rat Cyclic Guanosine Monophosphate ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 pmol/mL

Sensitivity

1.0 pmol/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pmol/mL

1 vial

STANDARD B (0.5mL)

5.0 pmol/mL

1 vial

STANDARD C (0.5mL)

10 pmol/mL

1 vial

STANDARD D (0.5mL)

25 pmol/mL

1 vial

STANDARD E (0.5mL)

50 pmol/mL

1 vial

STANDARD F (0.5mL)

100 pmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cGMP ELISA kit uses an anti-cGMP antibody and an cGMP-HRP conjugate in a competitive enzyme immunoassay method. cGMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cGMP antibody binding site between cGMP from samples and cGMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cGMP. Since the number of sites is limited, as more sites are occupied by cGMP from the sample, fewer sites are left to bind cGMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cGMP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cyclic Guanosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cGMP and analogues was observed.


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Summary of the Assay Procedure for Rat Cyclic Guanosine Monophosphate ELISA kit

Summary of the Assay Procedure for Rat Cyclic Guanosine Monophosphate ELISA kit

Citations of Rat Cyclic Guanosine Monophosphate ELISA kit

E02G0003 has been referenced in the below publications:

Design and Synthesis of a small peptide RWR containing RGD and initial exploration of function.

Vasodilatory effect of formaldehyde via the NO/cGMP pathway and the regulation of expression of KATP, BKCa and L-type Ca2+ channels.


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