E02C0010 Rat CytokeRatin Fragment Antigen 21.1 ELISA kit
The Rat CytokeRatin Fragment Antigen 21.1 ELISA kit can be used to identify samples from the rat species. CytokeRatin Fragment Antigen 21.1 can also be called fragment of cytokeratin 19 (CK19), CYFRA21.1.
Product Information | |
Cat. No. | E02C0010 |
Product Name | Rat CytokeRatin Fragment Antigen 21.1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CYFRA21.1 ELISA kit uses an anti-CYFRA21.1 antibody and an CYFRA21.1-HRP conjugate in a competitive enzyme immunoassay method. CYFRA21.1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CYFRA21.1 antibody binding site between CYFRA21.1 from samples and CYFRA21.1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CYFRA21.1. Since the number of sites is limited, as more sites are occupied by CYFRA21.1 from the sample, fewer sites are left to bind CYFRA21.1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYFRA21.1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CYFRA21.1 and analogues was observed. | |
E02C0010 has been referenced in the below publications：
The Effects of Radon and Its Daughter on Targeted Organs and Protective Effect of Radon-protection agent in Rats.
Effects of radon on the gene methylation in BALF cells and tumor markers in serum with rats as experimental mode.
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