E02D0001 Rat Dihydrotestosterone ELISA kit
The Rat Dihydrotestosterone ELISA kit can be used to identify samples from the rat species. Dihydrotestosterone can also be called DHT, 5α-Dihydrotestosterone, 5α-DHT, Androstanolone, Stanolone, 17β-Hydroxy-5α-androstan-3-one, 5α-Androstan-17β-ol-3-one.
Product Information | |
Cat. No. | E02D0001 |
Product Name | Rat Dihydrotestosterone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 nmol/L |
Sensitivity | 0.1 nmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 nmol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 nmol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 nmol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 nmol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 nmol/L | 1 vial |
STANDARD F (0.5mL) | 10 nmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DHT ELISA kit uses an anti-DHT antibody and an DHT-HRP conjugate in a competitive enzyme immunoassay method. DHT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DHT antibody binding site between DHT from samples and DHT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DHT. Since the number of sites is limited, as more sites are occupied by DHT from the sample, fewer sites are left to bind DHT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHT concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DHT and analogues was observed. | |
E02D0001 has been referenced in the below publications：
Physcion, a novel inhibitor of 5α-reductase that promotes hair growth in vitro and in vivo.
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