E02E0314 Rat Estrogens ELISA kit
The Rat Estrogens ELISA kit can be used to identify samples from the rat species. Estrogens can also be called ES, oestrogen.
Rat Estrogens ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
ES ELISA kit uses an anti-ES antibody and an ES-HRP conjugate in a competitive enzyme immunoassay method. ES-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ES antibody binding site between ES from samples and ES-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ES. Since the number of sites is limited, as more sites are occupied by ES from the sample, fewer sites are left to bind ES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ES concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between ES and analogues was observed.
E02E0314 has been referenced in the below publications：
Testosterone regulates myosin II isoforms expression and functional activity in the rat prostate.
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