E02I0350 Rat Inhibitor of kB kinase ELISA kit
The Rat Inhibitor of kB kinase ELISA kit can be used to identify samples from the rat species. Inhibitor of kB kinase can also be called IKK.
Specifications of Rat Inhibitor of kB kinase ELISA kit
Product Information | |
Cat. No. | E02I0350 |
Product Name | Rat Inhibitor of kB kinase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IKK ELISA kit uses an anti-IKK antibody and an IKK-HRP conjugate in a competitive enzyme immunoassay method. IKK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IKK antibody binding site between IKK from samples and IKK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IKK. Since the number of sites is limited, as more sites are occupied by IKK from the sample, fewer sites are left to bind IKK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKK concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Inhibitor of kB kinase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IKK and analogues was observed. | |
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Summary of the Assay Procedure for Rat Inhibitor of kB kinase ELISA kit
Citations of Rat Inhibitor of kB kinase ELISA kit
E02I0350 has been referenced in the below publications：
Comparative Analysis of NF-κB in the MyD88-Mediated Pathway After Implantation of Titanium Alloy and Stainless Steel and the Role of Regulatory T Cells.
