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BlueGene Biotech Rat Macrophage MigRation Inhibitory Factor ELISA kit

E02M0209 Rat Macrophage MigRation Inhibitory Factor ELISA kit

The Rat Macrophage MigRation Inhibitory Factor ELISA kit can be used to identify samples from the rat species. Macrophage MigRation Inhibitory Factor can also be called Macrophage Migration Inhibitory Factor, GIF, GLIF, Glycosylation inhibiting factor, MIF protein, MMIF, Phenylpyruvate tautomerase, acrophage migration inhibitory factor (glycosylation-inhibiting factor), L-dopachrome isomerase, MIF.

Specifications of Rat Macrophage MigRation Inhibitory Factor ELISA kit

Product Information

Cat. No.

E02M0209

Product Name

Rat Macrophage MigRation Inhibitory Factor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MIF ELISA kit uses an anti-MIF antibody and an MIF-HRP conjugate in a competitive enzyme immunoassay method. MIF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MIF antibody binding site between MIF from samples and MIF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MIF. Since the number of sites is limited, as more sites are occupied by MIF from the sample, fewer sites are left to bind MIF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIF concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Macrophage MigRation Inhibitory Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MIF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Macrophage MigRation Inhibitory Factor ELISA kit

Summary of the Assay Procedure for Rat Macrophage MigRation Inhibitory Factor ELISA kit

Citations of Rat Macrophage MigRation Inhibitory Factor ELISA kit

E02M0209 has been referenced in the below publications:

Therapeutic Effect of Simiaoyong'an Recipe Aqueous Extract and Its Influence on Peripheral Blood IL-1β,TNF-α and MIF Levels in Rats with Acute Gouty Arthritis.

中药熏洗治疗膝关节骨性关节炎的临床研究。

Study of circadian rhythm on anti--rheumatic in active ingredients of traditional Chinese medicine.


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