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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Platelet Factor 4 ELISA kit

E02P0085 Rat Platelet Factor 4 ELISA kit

The Rat Platelet Factor 4 ELISA kit can be used to identify samples from the rat species. Platelet Factor 4 can also be called C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, CXCL 4, CXCL4, Iroplact, Oncostatin A, Oncostatin-A, OncostatinA, PF-4, Platelet factor 4, SCYB 4, SCYB4, Small inducible cytokine subfamily member 4, PF4, PF 4.

Specifications of Rat Platelet Factor 4 ELISA kit

Product Information

Cat. No.

E02P0085

Product Name

Rat Platelet Factor 4 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PF4 ELISA kit uses an anti-PF4 antibody and an PF4-HRP conjugate in a competitive enzyme immunoassay method. PF4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PF4 antibody binding site between PF4 from samples and PF4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PF4. Since the number of sites is limited, as more sites are occupied by PF4 from the sample, fewer sites are left to bind PF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Platelet Factor 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PF4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Platelet Factor 4 ELISA kit

Summary of the Assay Procedure for Rat Platelet Factor 4 ELISA kit

Citations of Rat Platelet Factor 4 ELISA kit

E02P0085 has been referenced in the below publications:

Effect of different compatibility of huayu-tongluo Chinese herbs on the kidney of diabetic nephropathy rats the expression of integrin alpha 3 beta 1 and the related proteins in wnt/beta-catenin pathway.

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