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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

E02P0578 Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

The Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit can be used to identify samples from the rat species. Procollagen Type Ⅰ N Terminal Propeptide can also be called P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide.

Specifications of Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

Product Information

Cat. No.

E02P0578

Product Name

Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PINP ELISA kit uses an anti-PINP antibody and an PINP-HRP conjugate in a competitive enzyme immunoassay method. PINP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PINP antibody binding site between PINP from samples and PINP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PINP. Since the number of sites is limited, as more sites are occupied by PINP from the sample, fewer sites are left to bind PINP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PINP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PINP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

Summary of the Assay Procedure for Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

Citations of Rat Procollagen Type Ⅰ N Terminal Propeptide ELISA kit

E02P0578 has been referenced in the below publications:

The effects of estrogen and PTH on spinal Wnt/β-catenin pathway and degeneration in ovariectomized rats.

Estrogen alone or in combination with parathyroid hormone can decrease vertebral MEF2 and sclerostin expression and increase vertebral bone mass in ovariectomized rats.

The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.

Involvement of periostin-sclerostin-Wnt/β-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin.


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