E02O0020 Rat Osteoprotegerin ELISA kit
The Rat Osteoprotegerin ELISA kit can be used to identify samples from the rat species. Osteoprotegerin can also be called TNFRSF11B, TNFRSF11-B, OCIF, OPG, TR1, Tumor Necrosis Factor Receptor Superfamily Member 11b, Osteoclastogenesis Inhibitory Factor
Product Information | |
Cat. No. | E02O0020 |
Product Name | Rat Osteoprotegerin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OPG ELISA kit uses an anti-OPG antibody and an OPG-HRP conjugate in a competitive enzyme immunoassay method. OPG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OPG antibody binding site between OPG from samples and OPG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OPG. Since the number of sites is limited, as more sites are occupied by OPG from the sample, fewer sites are left to bind OPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OPG concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OPG and analogues was observed. | |
E02O0020 has been referenced in the below publications：
Effects of α-zeranol on expression of osteoprotegerin in bone marrow mesenchymal stem cells-derived osteoblasts.
Effects of combination of Tripterygium Glycoside with Ginsenoside on the proliferation and RANKL/OPG expression of rat fibroblast-like synoviocytes induced by Macrophage migration inhibitory factor.
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